Dual-Label Detection of Amplified Products in Quantitative RT-PCR Assay Using Lanthanide-Labeled Probes
نویسندگان
چکیده
منابع مشابه
Dual-label detection of amplified products in quantitative RT-PCR assay using lanthanide-labeled probes.
Quantitative RT-PCR (QRT-PCR) enables the sensitive and specific detection of mRNA with a small copy number. We used the QRT-PCR method and dual-label analysis of amplification products for the detection of prostate-specific antigen (PSA) mRNA. The QRT-PCR assay employed a PSA-like internal standard (IS) mRNA, which was used to quantify the PSA mRNA copies and to control the variations during t...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2001
ISSN: 0736-6205,1940-9818
DOI: 10.2144/01304rr01